Laboratory testing

HLA typing using Illumina NGS

We offer a HLA typing service for high-throughput projects. We have capacity to type large cohorts of samples to unambiguous (majority of cases) high resolution (min 4 digit resolution) in only a single pass.

We offer:

• Class 1 HLA A (Exon 2,3)
• B (Exon 2,3)
• C (Exon 2,3) and Class II DRB1,3,4,5 (Exon 2)
• DQB1 (Exon 2,3)
• DQA1 (Exon 2)
• DPB1 (Exon2)

exon specific typing that resolves Alleles to 4 or more digit typing levels covering coding region allelic variation. This can be further combined with other host specific genotyping as required at little extra cost. Our current cost for basic HLA typing in a commercial setting for small numbers of samples (<=50) is now $320 USD per sample that covers all the loci mentioned. For research and academic projects and for collaborative projects the discounts are significant. For further details and to get your project assessed for collaborative gain, and discounts please inquire. Discounts may be available for larger sample sets. There would be little if any discounts applied for only specific subset (Class I or Class II typing alone) unless very large sample numbers need to be processed. For population based HLA linkages sample numbers need to be carefully considered to get statistical significance in most study types. We are happy to advise in any given situation.

The nature of genetic testing and genetic variability may mean that in rare cases we may be unable to identify an Allele at a particular locus.

For pre-configured plates with ideal DNA concentrations in a first pass typing the turnaround times for <1000 samples are approximately 4 weeks from sample arrival. We recommend the following plates Axygen full or half skirted plates (Cat # PCR-96M2-HS-C or PCR-96-FS-C which are compatible with strip caps PCR-02CP-C). Plates must be sealed to prevent cross well contamination during shipping. Please leave wells H6 G12 and H12 empty  and fill order should be A1, B1, C1...etc if possible for us to add controls and avoid transpositions. We ask for ideally 50ul of 50ng/ul DNA with an A260/A280 of at least 1.7 and a size average of >12KB.  Lower amounts and quality can be processed but we cannot guarantee results due to possible allele dropout.

Alternative plates are acceptable please contact us at specimenreception@iiid.com.au if you wish to use alternative plates and layouts for fill order and control wells.  Failure to comply with the specific layout requirements may delay results.

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